Interleukin 3 (IL-3) is a hematopoietic growth factor with a broad spectrum of biologic activities. These include the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types.
MACS GMP Recombinant Human IL-3 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
IL-3 is mainly produced by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. Beside its role as hematopoietic growth factor, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.
Applications
MACS GMP Recombinant Human IL-3 can be used for a variety of applications, including the
ex vivo
cultivation of human plasmacytoid dendritic cells from enriched CD304 (BDCA-4)
+
cells.
2–4
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Kitamura
et al.1
using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human IL-3 (NIBSC code 91/510) provided by the National Institute for Biological Standards and Control.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-3. The peak corresponds to the calculated molecular mass of 15089 Da.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-3. The peak corresponds to the calculated molecular mass of 15089 Da.
Selected references
Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
Grouard, G. et al. (1997) The enigmatic plasmacytoid T cells develop into dendritic cells with interleukin (IL)-3 and CD40-ligand. J. Exp. Med. 185: 1101-1111
Colonna, M. et al. (2004) Plasmacytoid dendritic cells in immunity. Nat. Immunol. 5: 1219-1226
Cella, M. et al. (2000) Plasmacytoid dendritic cells activated by influenza virus and CD40L drive a potent TH1 polarization. Nat. Immunol. 1: 305-310
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