Granulocyte-macrophage colony–stimulating factor (GM-CSF) is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors.
MACS GMP Recombinant Human GM-CSF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
Beside its role as growth factor for granulocyte and monocyte precursors, GM-CSF also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Applications
MACS GMP Recombinant Human GM-CSF can be used for a variety of applications, including the
ex vivo
generation of human dendritic cells from enriched CD14
+
monocytes.
2–4
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Kitamura
et al.1
using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human GM-CSF (NIBSC code 88/646) provided by the National Institute for Biological Standards and Control.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human GM-CSF. The peak corresponds to the calculated molecular mass of 14474 Da.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human GM-CSF. The peak corresponds to the calculated molecular mass of 14474 Da.
Selected references
Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
Jonuleit et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142
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