Epidermal growth factor (EGF) is a small mitogenic polypeptide that stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, including fibroblasts, epithelial cells, and endothelial cells.
MACS
®
GMP Recombinant Human EGF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
EGF is the prototype of the large family of EGF-like proteins with a common structural motif comprising three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens.
In vitro
, EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, neural precursors, epithelial and endothelial cells, and promotes colony formation of epidermal cells.
Applications
MACS GMP Recombinant Human EGF can be used as an ingredient for many cell culture applications. EGF can support the maintenance of ectodermal and mesodermal progenitor cells, and stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Robinson and Gaines-Das
1
using 3T3 cells. The proliferation assay was calibrated with the international standard for human EGF (NIBSC code 91/530) provided by the National Institute for Biological Standards and Control.
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