Thrombopoietin (TPO), also termed megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone and the major stimulator of megakaryopoiesis and platelet production. It promotes the proliferation of hematopoietic stem cells, primitive progenitors, megakaryocytes, and platelets.
MACS GMP Recombinant Human TPO is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
TPO is expressed in liver, kidney, spleen, lung, bone marrow, and brain. The TPO receptor is a product of the proto-oncogene
c-mpl
and displays homology with type I cytokine receptor superfamily members. The receptor is present mainly on hematopoietic stem cells, megakaryocytic progenitors, megakaryocytes, and platelets.
Applications
MACS GMP Recombinant Human TPO can be used for a variety of applications, including the expansion
2,3
and the viral transduction
4,5
of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood, or the
in vitro
differentiation of megakaryocytes
6
.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Page
et al.1
using M07e cells. The proliferation assay was calibrated with the Non WHO Reference Material for human TPO (NIBSC code 03/124) provided by the National Institute for Biological Standards and Control.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TPO. The first RP-HPLC peak corresponds to the calculated molecular mass of 18804 Da with N-terminal methionine.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TPO. The first RP-HPLC peak corresponds to the calculated molecular mass of 18804 Da with N-terminal methionine.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TPO. The second RP-HPLC peak corresponds to the calculated molecular mass of 18832 Da with N-terminal formyl-methionine.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TPO. The second RP-HPLC peak corresponds to the calculated molecular mass of 18832 Da with N-terminal formyl-methionine.
MACS GMP Recombinant Human TPO biological activity.
Activity of Human TPO, GMP grade, (blue bar) was compared to another commercially available product (black bar).
MACS GMP Recombinant Human TPO biological activity.
Activity of Human TPO, GMP grade, (blue bar) was compared to another commercially available product (black bar).
Selected references
Page, L. A. et al. (1996) A sensitive human cell line based bioassay for megakaryocyte growth and development factor or thrombopoietin. Cytokine 8(1): 66-69
Kaushansky, K. and Drachman, J. G. (2002) The molecular and cellular biology of thrombopoietin: the primary regulator of platelet production. Oncogene 21(21): 3359-3367
) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.
J. Exp. Med. 183(6): 2551-2558
Kiem, H. P. et al. (1998) Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood 92(6): 1878-1886
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