Stem cell factor (SCF), also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF) is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration.
MACS GMP Recombinant Human SCF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. SCF is constitutively produced by endothelial cells and by stromal fibroblasts.
Applications
MACS GMP Recombinant Human SCF can be used for a variety of applications, including the expansion
2,3
and the viral transduction
4,5
of hematopoietic stem and progenitor cells derived from bone marrow, peripheral blood cells, and cord blood, or the
in vitro
differentiation of megakaryocytes
6
.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay according to Kitamura
et al.1
using TF-1 cells provided by the German Resource Centre for Biological Material (DSMZ). The proliferation assay was calibrated with the international standard for human SCF (NIBSC code 91/682) provided by the National Institute for Biological Standards and Control.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human SCF. The peak corresponds to the calculated molecular mass of 18585 Da.
Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human SCF. The peak corresponds to the calculated molecular mass of 18585 Da.
Selected references
Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
Broudy, V. C. (1997) Stem cell factor and hematopoiesis. Blood 90(4): 1345-1364
) human hematopoietic cells: novel responses to Flt3-ligand and thrombopoietin.
J. Exp. Med. 183(6): 2551-2558
Kiem, H. P. et al. (1998) Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development factor. Blood 92(6): 1878-1886
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