Interleukin 21 (IL-21) has pleiotropic effects on both cellular and humoral immune responses, such as stimulation of lymphocyte proliferation, promotion of CD8
+
T cell and NK cell cytotoxicity, and differentiation of B cells into plasma cells.
MACS GMP Recombinant Human IL-21 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for manufacture of this product. The product is lyophilized without carrier protein or preservatives.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
IL-21 is a four α-helix bundle cytokine and closely related to IL-2, IL-7, and IL-15. IL-21 expression is restricted to activated CD4
+
T helper cells and NKT cells. The functional receptor for IL-21, composed of the IL-21 receptor- and the common γ-chain, is expressed on various hematopoietic cells including T, B, NK and dendritic cells.
Applications
MACS GMP Recombinant Human IL-21 can be used for a variety of applications, including the
ex vivo
generation of antigen-specific cytotoxic T cells
1,2
or the
ex vivo
stimulation of NK cells
3
.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
Biological activity
The specific activity is determined by proliferation assay using mouse B9 hybridoma cells provided by the German Resource Centre for Biological Material (DSMZ).
IL-2 and IL-21 confer opposing differentiation programs to CD8
+
T cells for adoptive immunotherapy.
Blood 111: 5326-5333
de Rham, C. et al. (2007) The proinflammatory cytokines IL-2, IL-15 and IL-21 modulate the repertoire of mature human natural killer cell receptors. Arthritis Res. Ther. 9: R125
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