Alternative names

Technical specifications

• Antigen: CD117
• Clone: REAL338
• Isotype control: REAL Control
• Alternative names of antigen: c-kit, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, KIT, SCFR
• Distribution of antigen: hematopoietic stem cells, myeloid cells, red blood cells, mast cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, PE

• Figure 1

  Bone marrow cells from C57BL/6 mice were stained with REAlease CD117-APC (B) or with the corresponding REAL Control (A). REAlease CD117-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD117-APC (D). Cells were labeled with CD45R antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence

  A:	B:
  C:	D:

• Figure 2

  Bone marrow cells from C57BL/6 mice were stained with REAlease CD117 Complexes or with the corresponding REAL Control (left images) as well as with CD45R antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.