Alternative names

Technical specifications

• Antigen: CD8
• Clone: REAL100
• Isotype control: REAL Control
• Alternative names of antigen: Leu-2, CD8a, MAL, p32, Cd8b1, LY3, Lyt3, P37, Ly-2
• Distribution of antigen: T cells, thymocytes, NK cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, VioGreen

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD8-FITC (B) or with the corresponding REAL Control (A). REAlease CD8-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD8-FITC (D). Cells were labeled with CD3 antibodies in addition and were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD8 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Devine, L. et al. (1999) Orientation of the Ig domains of CD8 alpha beta relative to MHC class I. J. Immunol. 162(2): 846-851
  2. Gao, G. F. et al. (2000) Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunol. Today 21(12): 630-636