Alternative names

Technical specifications

• Antigen: CD14
• Clone: REAL107
• Isotype control: REAL Control
• Alternative names of antigen: LPS R
• Distribution of antigen: monocytes, macrophages
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, VioBlue, PerCP

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD14-FITC (B) or with the corresponding REAL Control (A). REAlease CD14-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD14-FITC (D). Cells were labeled with CD4 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD14 Complexes or with the corresponding REAL Control (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Goyer, S. M. and Ferrero, E. (1987) Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). Oxford, Oxford University Press
  2. Wilson, A. D. et al. (1995)
     Selection of monoclonal antibodies for the identification of lymphocyte surface antigens in the New World primate

     Saguinus oedipus oedipus

     (cotton top tamarin).
     J. Immunol. Methods 178: 195-200
  3. Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J. Immunol. 165: 6037-6046
  4. Neu, C. et al. (2013) CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages. PLoS One 8(6): e66898
  5. Paccou, J. et al. (2013)
     Determination and modulation of total and surface calcium-sensing receptor expression in monocytes

     in vivo

     and

     in vitro

     .
     PLoS One 8(10): e74800