Alternative names

Technical specifications

• Antigen: CD31
• Clone: REAL123
• Isotype control: REAL Control
• Alternative names of antigen: PECAM1, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, EndoCAM
• Distribution of antigen: endothelial cells, monocytes, platelets, granulocytes, lymphocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD31-FITC (B) or with the corresponding REAL Control (A). REAlease CD31-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD31-FITC (D). Cells were labeled with CD14 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Selected references

  1. Newman, P. J. et al. (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247(4947): 1219-1222
  2. Stockinger, H. et al. (1990) Molecular characterization and functional analysis of the leukocyte surface protein CD31. J. Immunol. 145(11): 3889-3897
  3. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794