Alternative names

Technical specifications

• Antigen: CD5
• Clone: REAL174
• Isotype control: REAL Control
• Alternative names of antigen: Ly-1, Ly-12, Ly-A, Lyt-1, T1, Tp67
• Distribution of antigen: thymocytes, T cells, B cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioGreen, PE

• Figure 1

  Splenocytes from Balb/c mice were stained with REAlease CD5-VioGreen™ (B) or with the corresponding REAL Control (A). REAlease CD5-VioGreen was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD5-VioGreen (D). Cells were labeled with CD3ε antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from Balb/c mice were stained with REAlease CD5 Complexes or with the corresponding REAL Control (left images) as well as with CD3ε antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Tarakhovsky, A. et al. (1995) A role for CD5 in TCR-mediated signal transduction and thymocyte selection. Science 269(5223): 535-537
  2. Efanov, A. et al. (2010)
     CD5

     ⁺

     CD23

     ⁺

     leukemic cell populations in TCL1 transgenic mice show significantly increased proliferation and Akt phosphorylation.
     Leukemia 24(5): 970-975
  3. Lundy, S. K. et al. (2014)
     Characterization and activity of Fas ligand producing CD5

     ⁺

     B cells.
     Methods Mol. Biol. 1190: 81-102