Alternative names

Technical specifications

• Antigen: CD49b
• Clone: REAL133
• Isotype control: REAL Control
• Alternative names of antigen: Itga2, gpIa, VLA-2α, VLA-2alpha, DX5
• Distribution of antigen: NK cells, NKT cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, FITC

• Figure 1

  Splenocytes from BALB/c mice were stained with REAlease CD49b-FITC (B) or with the corresponding REAL Control (A). REAlease CD49b-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD49b-FITC (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from C57BL/6 mice were stained with REAlease CD49b Complexes or with the corresponding REAL Control (left images) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Edelman, J. M. et al. (1994) The mouse VLA-2 homologue supports collagen and laminin adhesion but not virus binding. Cell Adhes. Commun. 2(2): 131-143
  2. Gagliani, N. et al. (2013) Coexpression of CD49b and LAG-3 identifies human and mouse T regulatory type 1 cells. Nat. Med. 19(6): 739-746
  3. Hanazawa, A. et al. (2013) CD49b/CD69-dependent generation of resting T helper cell memory. Front Immunol 4: 183