YILIMART - REAlease CD366 (TIM-3)-PE(REAL226) Kit,h

Clone REAL226 is an antibody fragment derived from the full CD366 (TIM-3) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL226 recognizes the human CD366 antigen, a single-pass type I membrane protein which is also known as T cell immunoglobulin and mucin domain containing protein-3 (TIM-3) or Hepatitis A virus cellular receptor 2 (HAVcr-2). CD366 is expressed on activated T cells, especially Tʜ1 cells, monocytes, macrophages, and dendritic cells. It is an activation-induced inhibitory molecule involved in tolerance and shown to induce T cell exhaustion in chronic viral infection and cancers. CD366 has been proposed to inhibit Tʜ1-mediated immune responses and regulates Tʜ1 and Tʜ17 cytokine secretion. The interaction of CD366 and its ligand galectin-9 induces apoptosis of Tʜ1 cells.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

HAVcr-2, TIMD-3

Technical specifications

Antigen: CD366 (TIM-3)
Clone: REAL226
Isotype control: REAL Control
Alternative names of antigen: HAVcr-2, TIMD-3
Distribution of antigen: dendritic cells, macrophages, monocytes, T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD366 (TIM-3)-PE (B) or with the corresponding REAL Control (A). REAlease CD366 (TIM-3)-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD366 (TIM-3)-PE (D). Cells were labeled with CD3 and CD4 antibodies in addition and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

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REAlease CD366 (TIM-3)-PE(REAL226) Kit,h

For research use only

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Alternative names

Technical specifications

Figure 1

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