YILIMART - REAlease CD133/1-APC (REAL233) Kit, h

Clone REAL233 is an antibody fragment derived from the full CD133/1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL233 recognizes the epitope 1 of the human CD133 antigen (CD133/1). This molecule is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34

^–

cells as well as on a subset of CD34

^bright

stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. CD133 has also been found to be expressed on circulating endothelial progenitor cells, fetal neural stem cells, other tissue-specific stem cells, such as renal, prostate, and corneal stem cells, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

PROM1, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4

Technical specifications

Antigen: CD133/1
Clone: REAL233
Isotype control: REAL Control
Alternative names of antigen: PROM1, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4
Distribution of antigen: brain, endothelial cells, epithelial cells, heart, hematopoietic stem cells, kidney, liver, lung, pancreas, placenta, ES and iPS cells, red blood cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD133/1-APC (B) or with the corresponding REAL Control (A). REAlease CD133/1-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD133/1-APC (D). Cells were labeled with CD34 antibodies in addition and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

REAlease CD133/1-APC (REAL233) Kit, h

For research use only

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Alternative names

Technical specifications

Figure 1

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