YILIMART - REAlease CD127-PE (REAL102) Kit, h

Clone REAL102 is an antibody fragment derived from the full CD127 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL102 recognizes the CD127 antigen, which is the α-chain of the interleukin 7 (IL-7) receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc). CD127 can be identified on immature B cells through the early pre–B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation. On regulatory T cells CD127 is absent and its expression is inversely correlated with FoxP3 expression and suppressive function. CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA

Technical specifications

Antigen: CD127
Clone: REAL102
Isotype control: REAL Control
Alternative names of antigen: IL-7R, IL-7Rα, IL-17Ralpha, CDW127, ILRA
Cross-reactivity:

cynomolgus monkey (
Macaca fascicularis
)

,

rhesus monkey (
Macaca mulatta
)
Distribution of antigen: B cells, T cells, thymocytes
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, PE, APC, PE-Vio770

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD127-APC (B) or left unstained (A). REAlease CD127-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD127-APC (D). Cells were labeled with CD4 antibodies in addition and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

Figure 2

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD127 Complexes or with the corresponding REAL Control (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Selected references
1. Armitage, R. J. et al. (1991) Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv. Exp. Med. Biol. 292: 121-130
2. Sudo, T. et al. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 90: 9125-9129
3. Fry, T. J. and Mackall, C. L. (2002) Interleukin-7: from bench to clinic. Blood 99: 3892-3904
4. Cupedo, T. et al. (2005) Development and activation of regulatory T cells in the human fetus. Eur. J. Immunol. 35: 383-390
5. Liu, S. et al. (2006) Hedgehog signaling and Bmi-1 regulate self-renewal of normal and malignant human mammary stem cells. Cancer Res. 66(12): 6063-6071
6. Seddiki, N. et al. (2006) Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J. Exp. Med. 203: 1693-1700

REAlease CD127-PE (REAL102) Kit, h

For research use only

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Alternative names

Technical specifications

Figure 1

Figure 2

Selected references

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