Alternative names

Technical specifications

• Antigen: CD90.1
• Clone: REAL505
• Isotype control: REAL Control
• Alternative names of antigen: THY1, T25, Thy-1, Thy-1.2, Thy-1.1
• Cross-reactivity: rat
• Distribution of antigen: brain, cancer stem cells, endothelial cells, fibroblasts, hematopoietic stem cells, leukemia cells, leukocytes, lymphocytes, mesenchymal stem cells, neurons, ES and iPS cells, T cells, thymocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PerCP, PE

• Figure 1

  Splenocytes from FVB mice were stained with REAlease CD90.1-PE (B) or with the corresponding REAL Control (A). REAlease CD90.1-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD90.1-PE (D). Cells were labeled with CD3ε antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from FVB mice were stained with REAlease CD90.1 Complexes or with the corresponding REAL Control (left images) as well as with CD3ε antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.