YILIMART - REAlease CD326 (EpCAM)-FITC, human

Clone REAL546 is an antibody fragment derived from the full CD326 (EpCAM) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL546 recognizes CD326, also known as human epithelial antigen (HEA), epithelial cell adhesion molecule (EpCAM), or epithelial-specific antigen (ESA), and is involved in cell adhesion. The CD326 antigen is broadly expressed on the basolateral surface of carcinoma and epithelial cells in tissues or on circulating tumor cells and cancer stem cells, but is not found on melanoma, neuroblastoma, sarcoma, lymphoma, leukemia cells, or normal fibroblasts. Furthermore, it is suggested, that CD326 could be used as a surface marker for human ESCs.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

EGP40, Ep-CAM, KSA, TROP1, MK-1

Technical specifications

Antigen: CD326 (EpCAM)
Clone: REAL546
Isotype control: REAL Control
Alternative names of antigen: EGP40, Ep-CAM, KSA, TROP1, MK-1
Distribution of antigen: cancer stem cells, epithelial cells, lung, ES and iPS cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC, PerCP, FITC

Figure 1

SK-BR-3 cells were stained with REAlease CD326 (EpCAM)-APC (B) or with the corresponding REAL Control (A). REAlease CD326 (EpCAM)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD326 (EpCAM)-APC (D). Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

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REAlease CD326 (EpCAM)-FITC, human

For research use only

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Alternative names

Technical specifications

Figure 1

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