YILIMART - CD49c-FITC, human, REAL349, 100 tests

Clone REAL349 is an antibody fragment derived from the full CD49c antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL349 recognizes the human CD49c antigen, a single-pass type I membrane protein also known as integrin α3 (ITGA3) or VLA-3α. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and plays a role in neural migration and corticogenesis, acted upon by such factors as netrin-1 and reelin. It is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. CD49c/CD29 plays a role in cell-cell and cell-matrix adhesion through binding fibronectin, laminin, collagen, epiligrin, thrombospondin, and CSPG4.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Integrin alpha-3 (ITGA3), MSK18, CD49 antigen-like family member C, FRP-2, Galactoprotein B3 (GAPB3), VLA-3 subunit alpha (VLA-3a)

Technical specifications

Antigen: CD49c
Clone: REAL349
Isotype control: REAL Control
Alternative names of antigen: Integrin alpha-3 (ITGA3), MSK18, CD49 antigen-like family member C, FRP-2, Galactoprotein B3 (GAPB3), VLA-3 subunit alpha (VLA-3a)
Distribution of antigen: endothelial cells, epithelial cells, fibroblasts
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, APC

Figure 1

A mixture of HeLa cell line and human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD49c-FITC (B) or with the corresponding REAL Control (A). REAlease CD49c-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD49c-FITC (D). Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

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CD49c-FITC, human, REAL349, 100 tests

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Alternative names

Technical specifications

Figure 1

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