Alternative names

Technical specifications

• Antigen: MSCA-1
• Clone: REAL219
• Isotype control: REAL Control
• Alternative names of antigen: ALPL, AP-TNAP, APTNAP, HOPS, TNAP, TNSALP
• Distribution of antigen: bone marrow, other
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Anti-MSCA-1-PE (B) or with the corresponding REAL Control (A). REAlease Anti-MSCA-1-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-MSCA-1-PE (D). Cells were labeled with CD45 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Anti-MSCA-1 Complexes or with the corresponding REAL Control (left images) as well as with CD45 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.