Technical specifications

• Antigen: CD45RO
• Clone: REAL377
• Isotype control: REAL Control
• Distribution of antigen: thymocytes, granulocytes, T cells, B cells, monocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD45RO-PE (B) or with the corresponding REAL Control (A). REAlease CD45RO-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD45RO-PE (D). Cells were labeled with CD45RA antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD45RO Complexes or with the corresponding REAL Control (left images) as well as with CD45RA antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.