Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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Thymocytes from BALB/c mice were stained with REAlease CD155-PE (B) or with the corresponding REAL Control (A). REAlease CD155-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD155-PE (D). Cells were labeled with CD8a antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence. |
A: | B: |
C: | D: |
Thymocytes from BALB/c mice were stained with REAlease CD155 Complexes or with the corresponding REAL Control (left images) as well as with CD8a antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence. |