Alternative names

Technical specifications

• Antigen: CD16/CD32
• Clone: REAL370
• Isotype control: REAL Control
• Alternative names of antigen: Fc-gamma-RIIB, FCGR3, FcRIII, FcR-10, Fc-gamma RII, FcRII, Ly-17
• Distribution of antigen: B cells, monocytes, NK cells, granulocytes, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, PE

• Figure 1

  Splenocytes from C57BL/6 mice were stained with REAlease CD16/CD32-PE (B) or with the corresponding REAL Control (A). REAlease CD16/CD32-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent, restained with REAlease CD16/CD32-PE (D), and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from C57BL/6 mice were stained with REAlease CD16/CD32 Complexes or with the corresponding REAL Control (left images) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.