YILIMART - Anti-Siglec-H-APC, mouse, REAL248, 100 t

Clone REAL248 is an antibody fragment derived from the full Siglec-H antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL248 recognizes the mouse siglec-H antigen, a 34 kDa transmembrane protein of the Ig superfamily known as mouse sialic acid-binding immunoglobulin-like lectin. Siglec-H is specifically expressed on mouse plasmacytoid dendritic cells, a subset of CD11c

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dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. Binding of antibodies to siglec-H inhibits type I interferon production, which can be induced in plasmacytoid dendritic cells by DNA and RNA viruses.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

SIGLECH

Technical specifications

Antigen: Siglec-H
Clone: REAL248
Isotype control: REAL Control
Alternative names of antigen: SIGLECH
Distribution of antigen: dendritic cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: APC

Figure 1

Splenocytes from C57BL6 mice were stained with REAlease Anti-Siglec-H-APC (B) or with the corresponding REAL Control (A). REAlease Anti-Siglec-H-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Siglec-H-APC (D). Cells were labeled with CD317 (PDCA-1) antibodies in addition and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

Selected references
1. Lund, J. et al. (2003) Toll-like receptor 9–mediated recognition of herpes simplex virus-2 by plasmacytoid dendritic cells. J. Exp. Med. 198(3): 513-520
2. Blasius, A. et al. (2004) A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 103(11): 4201-4206
3. Blasius, A. L. et al. (2006) Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 107(6): 2474-2476

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Anti-Siglec-H-APC, mouse, REAL248, 100 t

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Alternative names

Technical specifications

Figure 1

Selected references

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