YILIMART - CD370 (CLEC9A)-APC, m, REAL309, 100 t

Clone REAL309 is an antibody fragment derived from the full CD370 (CLEC9A) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL309 recognizes CD370 C-type lectin 9A (CLEC9A), also known as DC NK lectin group receptor-1 (DNGR-1). It is a type II transmembrane glycoprotein of 264 amino acids expressed at high level on CD8a

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dendritic cells and at lower level on mPDCA-1

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plasmacytoid dendritic cells. CD370 (CLEC9A) recognizes necrotic cell associated epitopes and plays an important role in crosspresentation of dead cell derived antigens.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

DNGR-1, CLEC9A

Technical specifications

Antigen: CD370 (CLEC9A)
Clone: REAL309
Isotype control: REAL Control
Alternative names of antigen: DNGR-1, CLEC9A
Distribution of antigen: dendritic cells, other
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: APC

Figure 1

A mixture of thymocytes from C57BL/6 mice and CLEC9A transfected cells was stained with REAlease CD370 (CLEC9A)-APC (B) or with the corresponding REAL Control (A). REAlease CD370 (CLEC9A)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD370 (CLEC9A)-APC (D). Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

CD370 (CLEC9A)-APC, m, REAL309, 100 t

For research use only

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