Alternative names

Technical specifications

• Antigen: CD45RA
• Clone: REAL164
• Isotype control: REAL Control
• Alternative names of antigen: Ptprc, B220, CD45, CD45R, GP180, L-CA, LY5, T200
• Cross-reactivity:
  cynomolgus monkey (

  Macaca fascicularis

  )
  ,
  rhesus monkey (

  Macaca mulatta

  )
• Distribution of antigen: T cells, B cells, NK cells, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, FITC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD45RA-FITC (B) or with the corresponding REAL Control (A). REAlease CD45RA-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD45RA-FITC (D). Cells were labeled with CD45RO antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD45RA Complexes or with the corresponding REAL Control (left images) as well as with CD45RO antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Cantisan, S. et al. (2010)
     CD45RA expression on HCMV-specific effector memory CD8

     ⁺

     T cells is associated with the duration and intensity of HCMV replication after transplantation.
     Clin. Immunol. 137(1): 81-88
  2. Baba, N. et al. (2010)
     Interplay between CD45RA

     ⁺

     regulatory T cells and TNF-α in the regulation of human Tʜ17 differentiation.
     Int. Immunol. 22: 237-244
  3. Braun, T. et al. (2012)
     Effector CD4

     ⁺

     CD45RA

     ^–

     CD25

     ^bright

     Foxp3

     ^bright

     regulatory T cell (eTreg) distribution is significantly impaired in chronic myelomonocytic leukemia (CMML) and correlates with TET 2 mutational status.
     Blood 120: 2808-2808