Alternative names

Technical specifications

• Antigen: CD41/CD61
• Clone: REAL179
• Isotype control: REAL Control
• Alternative names of antigen: gpIIb/IIIa complex, integrin αIIbβ3
• Distribution of antigen: megakaryocytes, platelets
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, VioGreen

• Figure 1

  Human peripheral blood cells after erythrocyte lysis were stained with REAlease CD41/CD61-VioGreen™ (B) or with the corresponding REAL Control (A). REAlease CD41/CD61-VioGreen was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD41/CD61-VioGreen (D). Cells were labeled with CD42a antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood cells after erythrocyte lysis were stained with REAlease CD41/CD61 Complexes or with the corresponding REAL Control (left images) as well as with CD42a antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Naik, U. P. et al. (1997) Identification of a novel calcium-binding protein that interacts with the integrin alphaIIb cytoplasmic domain. J. Biol. Chem. 272(8): 4651-4654
  2. Corbel, C. et al. (2005) (alpha)IIb integrin, a novel marker for hemopoietic progenitor cells. Int. J. Dev. Biol. 49(2-3): 279-284
  3. Podolnikova, N. P. et al. (2014) The interaction of integrin αIIbβ3 with fibrin occurs through multiple binding sites in the αIIb β-propeller domain. J. Biol. Chem. 289(4): 2371-2383