Alternative names

Technical specifications

• Antigen: CD56
• Clone: REAL161
• Isotype control: REAL Control
• Alternative names of antigen: NCAM1, MSK39, NCAM, Leu-19, NKH-1
• Cross-reactivity:
  rhesus monkey (

  Macaca mulatta

  )
  ,
  cynomolgus monkey (

  Macaca fascicularis

  )
• Distribution of antigen: NK cells, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, FITC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD56-PE (B) or with the corresponding REAL Control (A). REAlease CD56-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD56-PE (D). Cells were labeled with CD3 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD56 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Carter, D. L. et al. (1999)
     CD56 identifies monocytes and not natural killer cells in

     Rhesus macaques

     .
     Cytometry 37: 41-50
  2. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice. PLoS One 6(3): e17454
  3. Stockholm, D. et al. (2011) Bistable cell fate specification as a result of stochastic fluctuations and collective spatial cell behaviour. PLoS One 5(12): e14441