YILIMART - aMHC Class II (I-Ab)-PE,m,REAL362, 100 t

Clone REAL362 is an antibody fragment derived from the full MHC Class II (I-Ab) antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL362 recognizes the mouse MHC class II alloantigen I-Ab of H-2b bearing mouse strains. It also cross-reacts with the H-2k and H-2u haplotypes, but reactivity to other haplotypes like, for example, d, f, g7, p, q, r, and s has not been observed. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

MHC class II IAb

Technical specifications

Antigen: MHC Class II (I-Ab)
Clone: REAL362
Isotype control: REAL Control
Alternative names of antigen: MHC class II IAb
Distribution of antigen: B cells, dendritic cells, hematopoietic stem cells, macrophages, monocytes, thymic epithelial cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE

Figure 1

Splenocytes from C57BL6 mice were stained with REAlease Anti-MHC Class II (I-Ab)-PE (B) or with the corresponding REAL Control (A). REAlease Anti-MHC Class II (I-Ab)-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-MHC Class II (I-Ab)-PE (D). Cells were labeled with CD45R antibodies in addition and analyzed by flow cytometry using the MACSQuant

_^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

aMHC Class II (I-Ab)-PE,m,REAL362, 100 t

For research use only

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