YILIMART - Anti-TcR Vd4-PE, mouse, REAL353, 100 t

Clone REAL353 is an antibody fragment derived from the full TCR Vδ4 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL353 recognizes the mouse V delta 4 T cell receptor (TCR Vδ4) antigen, which is the predominant TCR of γ/δ T cells in the intestinal epithelium. The γ/δ TCR is expressed by a small subset of T cells in the thymus, intestinal epithelium, spleen, lymph node, and epidermis.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Technical specifications

Antigen: TCR Vδ4
Clone: REAL353
Isotype control: REAL Control
Distribution of antigen: T cells, spleen, lymph node
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE

Figure 1

Splenocytes from C57BL6 mice were stained with REAlease Anti-TCR Vδ4-PE (B) or with the corresponding REAL Control (A). REAlease Anti-TCR Vδ4-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-TCR Vδ4-PE (D). Cells were labeled with CD3ε antibodies in addition and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

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Anti-TcR Vd4-PE, mouse, REAL353, 100 t

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Technical specifications

Figure 1

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