Alternative names

Technical specifications

• Antigen: CD3
• Clone: REAL104
• Isotype control: REAL Control
• Alternative names of antigen: CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE
• Distribution of antigen: T cells, NK cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE, VioBlue, VioGreen, PerCP

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD3-FITC (B) or with the corresponding REAL Control (A). REAlease CD3-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD3-FITC (D). Cells were labeled with CD4 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD3 Complexes or with the corresponding REAL Control (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Gold, D. P. et al. (1986) Isolation of cDNA clones encoding the 20K non-glycosylated polypeptide chain of the human T-cell receptor/T3 complex. Nature 321(6068): 431-434
  2. D'Acquisto, F. et al. (2011)
     CD3

     ⁺

     CD4

     ^-

     CD8

     ^-

     (double negative) T cells: saviours or villains of the immune response?
     Biochem. Pharmacol. 82(4): 333-340
  3. Birnbaum, M. E. et al. (2014) Molecular architecture of the αβ T cell receptor-CD3 complex. Proc. Natl. Acad. Sci. U.S.A. 111(49): 17576-17581