Alternative names

Technical specifications

• Antigen: Ly-49C/I
• Clone: REAL296
• Isotype control: REAL Control
• Alternative names of antigen: Klra3, Klra9
• Distribution of antigen: NK cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, PE

• Figure 1

  Splenocytes from C56BL/6 mice were stained with REAlease Anti-Ly-49C/I-APC (B) or with the corresponding REAL Control (A). REAlease Anti-Ly-49C/I-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Ly-49C/I-APC (D). Cells were labeled with CD49b antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from C56BL/6 mice were stained with REAlease Anti-Ly-49C/I Complexes or with the corresponding REAL Control (left images) as well as with CD49b antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Stoneman, E. R. et al. (1995) Cloning and characterization of 5E6(Ly-49C), a receptor molecule expressed on a subset of murine natural killer cells. J. Exp. Med. 182(2): 305-313
  2. Brennan, J. et al. (1996) Heterogeneity among Ly-49C natural killer (NK) cells: characterization of highly related receptors with differing functions and expression patterns. J. Exp. Med. 184(6): 2085-2090
  3. Yu, Y. Y. et al. (1996) The role of Ly49A and 5E6(Ly49C) molecules in hybrid resistance mediated by murine natural killer cells against normal T cell blasts. Immunity 4(1): 67-76