Alternative names

Technical specifications

• Antigen: CD10
• Clone: REAL318
• Isotype control: REAL Control
• Alternative names of antigen: MME, CALLA, NEP, SFE, gp100, Neprilysin, Enkephalinase
• Distribution of antigen: B cells, bone marrow, brain, epithelial cells, fibroblasts, granulocytes, gut, kidney, leukemia cells, ES and iPS cells, smooth muscle, stromal cells, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC, VioBlue, FITC

• Figure 1

  A mixture of REH cells and human peripheral blood mononuclear cells (PBMCs) was stained with REAlease CD10-PE (B) or with the corresponding REAL Control (A). REAlease CD10-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD10-PE (D). Cells were labeled with CD19 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  A mixture of REH cells and human peripheral blood mononuclear cells (PBMCs) was stained with REAlease CD10 Complexes or with the corresponding REAL Control (left images) as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.