YILIMART - Anti-Ly49E/F-PE, mouse, REAL331, 100 t

Clone REAL331 is an antibody fragment derived from the full Ly-49E/F antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL331 recognizes the mouse killer cell lectin-like receptor 5 and 6 (Ly-49E and Ly-49F) antigens, which are type II transmembrane natural killer cell receptors belonging to the Ly-49 family. Members of the Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands, and include activating and inhibitory receptor molecules. Ly-49E is expressed abundantly on fetal or immature natural killer (NK) cells and on certain γδ T cells, but at barely detectable frequencies on adult splenic NK cells. Different from other Ly-49 receptors, Ly-49E contains an immunoreceptor tyrosine-based inhibitory motif domain, a charged transmembrane residue and it is present in all mouse strains. Its failure to bind to a variety of soluble or cell bound MHC class I molecules indicates that its natural ligand is not an endogenous class I molecule. Interleukin 2 (IL-2) and IL-15 selectively induce the

de novo

expression of Ly-49E on the majority of mature NK cells. By contrast, IL-4 blocks this IL-2 induced acquisition of Ly-49E. Ly-49F is expressed on subsets of NK cells, NK-T cells, and on a subpopulation of memory T cells in C57BL/6 mice, but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice. IL-21 selectively down-regulates Ly-49F on mature NK cells.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

Klra5, Ly49e, Ly49F, Klra6

Technical specifications

Antigen: Ly-49E/F
Clone: REAL331
Isotype control: REAL Control
Alternative names of antigen: Klra5, Ly49e, Ly49F, Klra6
Distribution of antigen: NK cells, T cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE

Figure 1

Splenocytes from C57BL6 mice were stained with REAlease Anti-Ly-49E/F-PE (B) or with the corresponding REAL Control (A). REAlease Anti-Ly-49E/F-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Ly-49E/F-PE (D). Cells were labeled with Anti-NK1.1 antibodies in addition and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

Anti-Ly49E/F-PE, mouse, REAL331, 100 t

For research use only

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Alternative names

Technical specifications

Figure 1

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