Alternative names

Technical specifications

• Antigen: CD115
• Clone: REAL272
• Isotype control: REAL Control
• Alternative names of antigen: Csfmr, Fim-2, FMS, M-CSFR, CSF-1R, c-fms
• Distribution of antigen: macrophages, monocytes, osteoclasts, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC, PE

• Figure 1

  Bone marrow cells from C57BL/6 mice were stained with REAlease CD115-PE (B) or with the corresponding REAL Control (A). REAlease CD115-PE was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD115-PE (D). Cells were labeled with Anti-Ly-6C antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Selected references

  1. Sudo, T. et al. (1995) Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene 11: 2469-2476
  2. Murayama, T. et al. (1999) Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation 99: 1740-1746
  3. Sunderkötter, C. et al. (2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response. J. Immunol. 172: 4410-4417
  4. Auffray, C. et al. (2009)
     CX

     ₃

     CR1

     ⁺

     CD115

     ⁺

     CD135

     ⁺

     common macrophage/DC precursors and the role of CX

     ₃

     CR1 in their response to inflammation.
     J. Exp. Med. 206(3): 595-606
  5. Breslin W. L. et al. (2013) Mouse blood monocytes: Standardizing their identification and analysis using CD115. J. Immunol. Methods 390(1-2): 1-8