Alternative names

Technical specifications

• Antigen: CD8a
• Clone: REAL137
• Isotype control: REAL Control
• Alternative names of antigen: CD8, Leu-2, Ly-2, T8, Lyt-2, Ly-35
• Distribution of antigen: T cells, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, VioBlue, VioGreen, FITC

• Figure 1

  Splenocytes from mice were stained with REAlease CD8a-FITC (B) or with the corresponding REAL Control (A). REAlease CD8a-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD8a-FITC (D). Cells were labeled with CD4 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from mice were stained with REAlease CD8a Complexes or with the corresponding REAL Control (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Nakauchi, H. et al. (1985) Molecular cloning of Lyt-2, a membrane glycoprotein marking a subset of mouse T lymphocytes: molecular homology to its human counterpart, Leu-2/T8, and to immunoglobulin variable regions. Proc. Natl. Acad. Sci. U.S.A. 82(15): 5126-5130
  2. Liaw, C. W. et al. (1986) Structure, sequence, and polymorphism of the Lyt-2 T cell differentiation antigen gene. J. Immunol. 137(3): 1037-1043
  3. Nakauchi, H. et al. (1987) Isolation and characterization of the gene for the murine T cell differentiation antigen and immunoglobulin-related molecule, Lyt-2. Nucleic Acids Res. 15(10): 4337-4347