Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD42b-APC (B) or with the corresponding REAL Control (A). REAlease CD42b-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD42b-APC (D). Cells were labeled with CD61 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence. |
A: | B: |
C: | D: |
Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD42b Complexes or with the corresponding REAL Control (left images) as well as with CD61 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence. |