Alternative names

Technical specifications

• Antigen: CD13
• Clone: REAL312
• Isotype control: REAL Control
• Alternative names of antigen: ANPEP, APN, gp150, LAP1, p150, PEPN
• Distribution of antigen: granulocytes, myeloid cells, endothelial cells, epithelial cells, lymphocytes, kidney, placenta
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: APC

• Figure 1

  Human peripheral blood cells after erythrocyte lysis were stained with REAlease CD13-APC (B) or with the corresponding REAL Control (A). REAlease CD13-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD13-APC (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Selected references

  1. Look, A. T. et al. (1989) Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N. J. Clin. Invest. 83(4): 1299-1307
  2. Yeager, C. L. et al. (1992) Human aminopeptidase N is a receptor for human coronavirus 229E. Nature 357(6377): 420-422
  3. Favaloro, E. J. et al. (1993) CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated. Exp. Hematol. 21(13): 1695-1701