YILIMART - CD200R3-APC, mouse, REAL249, 100 tests

Clone REAL249 is an antibody fragment derived from the full CD200R3 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL249 recognizes the human CD200R3 antigen. CD200R is an approximately 33 kDa single-pass type I membrane-protein which is a disulfide-linked dimer on the cell surface. It is reported, that the four different isoforms of CD200R (CD200R1, CD200R2, CD200R3, CD200R4) bind to CD200 (OX-2) as ligand. CD200 is primarily expressed on myeloid cells but also expressed on T and B lymphocytes. The combination of CD200R and CD200 inhibits mast cell degranulation, promotes macrophage activation, and decreases the activation of lymphocytes.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

OX-2 receptor 3

Technical specifications

Antigen: CD200R3
Clone: REAL249
Isotype control: REAL Control
Alternative names of antigen: OX-2 receptor 3
Distribution of antigen: T cells, B cells, myeloid cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: APC

Figure 1

Bone marrow cells from C57BL/6 mice were stained with REAlease CD200R3-APC (B) or with the corresponding REAL Control (A). REAlease CD200R3-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD200R3-APC (D). Cells were labeled with CD49b antibodies in addition and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

Selected references
1. Wright, G. J. et al. (2003) Characterization of the CD200 receptor family in mice and humans and their interactions with CD200. J. Immunol. 171: 3034-3046
2. Gorczynski, R. et al. (2004) CD200 is a ligand for all members of the CD200R family of immunoregulatory molecules. J. Immunol. 172: 7744-7749
3. Rijkers, E. S. et al. (2008) The inhibitory CD200R is differentially expressed on human and mouse T and B lymphocytes. Mol. Immunol. 45(4): 1126-1135

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CD200R3-APC, mouse, REAL249, 100 tests

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Alternative names

Technical specifications

Figure 1

Selected references

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