Alternative names

Technical specifications

• Antigen: CD185 (CXCR5)
• Clone: REAL284
• Isotype control: REAL Control
• Alternative names of antigen: CXCR5, BLR1, MDR15
• Distribution of antigen: B cells, monocytes, neurons, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD185 (CXCR5)-APC (B) or with the corresponding REAL Control (A). REAlease CD185 (CXCR5)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD185 (CXCR5)-APC (D). Cells were labeled with CD3 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD185 (CXCR5) Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Förster, R. et al. (1994) Expression of the G-protein-coupled receptor BLR1 defines mature, recirculating B cells and a subset of T-helper memory cells. Blood 84: 830-840
  2. Förster, R. et al. (1996) A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen. Cell 87(6): 1037-1047
  3. Saeki, H. et al. (2000)
     A migratory population of skin-derived dendritic cells expresses CXCR5, responds to B lymphocyte chemoattractant

     in vitro

     , and co-localizes to B cell zones in lymph nodes

     in vivo

     .
     Eur. J. Immunol. 30(10): 2808-2814
  4. Bryant, V. L. et al. (2007)
     Cytokine-mediated regulation of human B cell differentiation into Ig-secreting cells: predominant role of IL-21 produced by CXCR5

     ⁺

     T follicular helper cells.
     J. Immunol. 179: 8180-8190
  5. Chevalier, N. et al. (2011) CXCR5 expressing human central memory CD4 T cells and their relevance for humoral immune responses. J. Immunol. 186(10): 5556-5568
  6. Farooq, F. et al. (2016) Circulating follicular T helper cells and cytokine profile in humans following vaccination with the rVSV-ZEBOV Ebola vaccine. Sci. Rep. 6: 27944