Alternative names

Technical specifications

• Antigen: TCR Vδ1
• Clone: REAL277
• Isotype control: REAL Control
• Alternative names of antigen: TRDV1, hDV101S1
• Distribution of antigen: T cells, epithelial cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Anti-TCR Vδ1-FITC (B) or with the corresponding REAL Control (A). REAlease Anti-TCR Vδ1-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-TCR Vδ1-FITC (D). Cells were labeled with CD3 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Anti-TCR Vδ1 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Pepin, L. F. et al. (1993) Preferential V delta 1 expression among TcR gamma/delta-bearing T cells in human oral epithelium. Scand. J. Immunol. 37(3): 289-294
  2. Libero, G. D. et al. (1997) Sentinel function of broadly reactive human gamma delta T cells. Immunol. Today 18(1): 22-26
  3. Di Marco Barros, R. et al. (2016) Epithelia use butyrophilin-like molecules to shape organ-specific γδ T cell compartments. Cell 167(1): 203-218
  4. Zumwalde, N. A. et al. (2016)
     Analysis of immune cells from human mammary ductal epithelial organoids reveals Vδ2

     ⁺

     T cells that efficiently target breast carcinoma cells in the presence of bisphosphonate.
     Cancer Prev Res (Phila) 9: 305-316