Alternative names

Technical specifications

• Antigen: CD159a (NKG2A)
• Clone: REAL283
• Isotype control: REAL Control
• Alternative names of antigen: KLRC1, CD159a, NKG2, NKG2A
• Distribution of antigen: NK cells, T cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE, APC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD159a (NKG2A)-APC (B) or with the corresponding REAL Control (A). REAlease CD159a (NKG2A)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD159a (NKG2A)-APC (D). Cells were labeled with CD56 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD159a (NKG2A) Complexes or with the corresponding REAL Control (left images) as well as with CD56 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Boyington, J. C. et al. (1999) Structure of CD94 reveals a novel C-type lectin fold: implications for the NK cell-associated CD94/NKG2 receptors. Immunity 10(1): 75-82
  2. Sullivan, L. C. et al. (2007) The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 27(6): 900-911
  3. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
  4. Veluchamy, J. P. et al. (2017) High-efficiency lysis of cervical cancer by allogeneic NK cells derived from umbilical cord progenitors is independent of HLA status. Cancer Immunol. Immunother. 66: 51-61
  5. Jacomet, F. et al. (2017)
     The hypothesis of the human iNKT/innate CD8

     ⁺

     T-cell axis applied to cancer: evidence for a deficiency in chronic myeloid leukemia.
     Front Immunol 7: 688