YILIMART - Anti-TCR Vd1-FITC, human, REAL277, 100 t

Clone REAL277 is an antibody fragment derived from the full TCR Vδ1 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL277 recognizes the Vδ1 chain of γδ T cell receptor (TCR). The TCR δ locus is located within the TCR α locus on chromosome 14, six Vδ segments have been reported so far. γδ T cells comprise a small population of T cells and unlike peripheral circulating αβ T cells, γδ T cells are mainly found in body tissues such as thymus, peripheral lymphoid tissues, intestinal epithelium, and epidermis. Distribution analysis in human oral cavity revealed preferential localisation of Vδ 1+ γδ T cells in intraepithelial region.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

TRDV1, hDV101S1

Technical specifications

Antigen: TCR Vδ1
Clone: REAL277
Isotype control: REAL Control
Alternative names of antigen: TRDV1, hDV101S1
Distribution of antigen: T cells, epithelial cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, PE

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Anti-TCR Vδ1-FITC (B) or with the corresponding REAL Control (A). REAlease Anti-TCR Vδ1-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-TCR Vδ1-FITC (D). Cells were labeled with CD3 antibodies in addition and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

Figure 2

Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease Anti-TCR Vδ1 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Selected references
1. Pepin, L. F. et al. (1993) Preferential V delta 1 expression among TcR gamma/delta-bearing T cells in human oral epithelium. Scand. J. Immunol. 37(3): 289-294
2. Libero, G. D. et al. (1997) Sentinel function of broadly reactive human gamma delta T cells. Immunol. Today 18(1): 22-26
3. Di Marco Barros, R. et al. (2016) Epithelia use butyrophilin-like molecules to shape organ-specific γδ T cell compartments. Cell 167(1): 203-218
4. Zumwalde, N. A. et al. (2016)
  
  Analysis of immune cells from human mammary ductal epithelial organoids reveals Vδ2
  ⁺
  T cells that efficiently target breast carcinoma cells in the presence of bisphosphonate.
  
  Cancer Prev Res (Phila) 9: 305-316

Anti-TCR Vd1-FITC, human, REAL277, 100 t

For research use only

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Figure 1

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