YILIMART - CD16-VioGreen, human, REAL234, 100 tests

Clone REAL234 is an antibody fragment derived from the full CD16 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL234 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REAL234 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes.

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII

Technical specifications

Antigen: CD16
Clone: REAL234
Isotype control: REAL Control
Alternative names of antigen: FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII
Distribution of antigen: NK cells, macrophages, mast cells
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioGreen, PerCP, VioBlue

Figure 1

Human peripheral blood cells after erythrocyte lysis were stained with REAlease CD16-VioGreen™ (B) or with the corresponding REAL Control (A). REAlease CD16-VioGreen was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD16-VioGreen (D). Cells were labeled with CD15 antibodies in addition and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

A:	B:

C:	D:

CD16-VioGreen, human, REAL234, 100 tests

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Technical specifications

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