Alternative names

Technical specifications

• Antigen: CD45
• Clone: REAL180
• Isotype control: REAL Control
• Alternative names of antigen: ICAM-1, Ly-47, MALA-2
• Distribution of antigen: monocytes, macrophages, lymphocytes, endothelial cells, granulocytes, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioBlue, VioGreen, PerCP, FITC

• Figure 1

  Splenocytes from Balb/c mice were stained with REAlease CD45-FITC (B) or with the corresponding REAL Control (A). REAlease CD45-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD45-FITC (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Splenocytes from Balb/c mice were stained with REAlease CD45 Complexes or with the corresponding REAL Control (left images) as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Siu, G. et al. (1989) Isolation of the murine intercellular adhesion molecule 1 (ICAM-1) gene. ICAM-1 enhances antigen-specific T cell activation. J. Immunol. 143(11): 3813-3820
  2. Fine, J. S. et al. (1991) The role of LFA-1/ICAM-1 interactions during murine T lymphocyte development. J. Immunol. 147(9): 2852-2859
  3. Driessens, M. H. et al. (1996) Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct. J. Leukoc. Biol. 60(6): 758-765