Alternative names

Technical specifications

• Antigen: CD104 (Integrin β4)
• Clone: REAL151
• Isotype control: REAL Control
• Alternative names of antigen: Itgb4
• Distribution of antigen: epithelial cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, VioBlue, VioGreen

• Figure 1

  HT-29 cells were stained with REAlease CD104 (Integrin β4)-FITC (B) or with the corresponding REAL Control (A). REAlease CD104 (Integrin β4)-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD104 (Integrin β4)-FITC (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  HT-29 cells were stained with REAlease CD104 (Integrin β4) Complexes or with the corresponding REAL Control (left images) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Tuckwell, D. S. et al. (1993) Integrins: a review of their structure and mechanisms of ligand binding. Symp. Soc. Exp. Biol 47: 107-136
  2. Simon-Assmann, P. et al. (1994) Differential expression of laminin isoforms and alpha 6-beta 4 integrin subunits in the developing human and mouse intestine. Dev. Dyn. 201(1): 71-85