Alternative names

Technical specifications

• Antigen: CD45
• Clone: REAL258
• Isotype control: REAL Control
• Alternative names of antigen: Ptprc, GP180, L-CA, LY5, T200
• Distribution of antigen: T cells, B cells, leukocytes
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, PE, APC, VioGreen, PerCP, VioBlue

• Figure 1

  Human peripheral blood cells after erythrocyte lysis were stained with REAlease CD45-VioGreen™ (B) or with the corresponding REAL Control (A). REAlease CD45-VioGreen was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD45-VioGreen (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood cells after erythrocyte lysis were stained with REAlease CD45 Complexes or with the corresponding REAL Control (left images) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Ralph, S. J. et al. (1987) Structural variants of human T200 glycoprotein (leukocyte-common antigen). EMBO J. 6(5): 1251-1257
  2. Vykoukal, J. et al. (2008) Enrichment of putative stem cells from adipose tissue using dielectrophoretic field-flow fractionation. Lab Chip 8(8): 1386-1393
  3. Kurian, L. et al. (2013) Conversion of human fibroblasts to angioblast-like progenitor cells. Nat. Methods 10(1): 77-83