Technical specifications

• Antigen: SSEA-4
• Clone: REAL232
• Isotype control: REAL Control
• Cross-reactivity: rabbit, mouse, canine, chicken
• Distribution of antigen: ES and iPS cells, embryonic germ cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC, VioGreen

• Figure 1

  A mixture of NTERA-2 and U937 cells was stained with REAlease Anti-SSEA-4-FITC (B) or with the corresponding REAL Control (A). REAlease Anti-SSEA-4-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-SSEA-4-FITC (D). Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI).

  A:	B:
  C:	D:

• Figure 2

  A mixture of NTERA-2 and U937 cells was stained with REAlease Anti-SSEA-4 Complexes or with the corresponding REAL Control (left images) and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI).

• Selected references

  1. Kannagi, R. et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside EMBO J. 2(12): 2355-2361
  2. Gang, E. J. et al. (2007) SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood 109(4): 1743-1751
  3. Truong, T. T. et al. (2011) SSEA4 is a potential negative marker for the enrichment of human corneal epithelial stem/progenitor cells. Invest. Ophthalmol. Vis. Sci. 52(9): 6315-6320