Alternative names

Technical specifications

• Antigen: CD44
• Clone: REAL259
• Isotype control: REAL Control
• Alternative names of antigen: CD44s, EMCR III, H-CAM, Pgp-1
• Distribution of antigen: cancer stem cells, fibroblasts, hematopoietic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioBlue, VioGreen, FITC

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD44-VioGreen™ (B) or with the corresponding REAL Control (A). REAlease CD44-VioGreen was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD44-VioGreen (D). Cells were labeled with CD3 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD44 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Aruffo, A. et al. (1990) CD44 is the principal cell surface receptor for hyaluronate. Cell 61: 1303-1313
  2. Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
  3. Collins, A. T. et al. (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65: 10946-10951
  4. Patrawala, L. et al. (2006)
     Highly purified CD44

     ⁺

     prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells.
     Oncogene 25: 1696-1708
  5. Dalerba, P. et al. (2007) Phenotypic characterization of human colorectal cancer stem cells. Proc. Natl. Acad. Sci. U.S.A. 104: 10158-10163