Alternative names

Technical specifications

• Antigen: CD4
• Clone: REAL103
• Isotype control: REAL Control
• Alternative names of antigen: CD4mut, L3T4, Leu-3, T4
• Distribution of antigen: T helper cells, monocytes, dendritic cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: VioGreen, PerCP, FITC, PE, APC, VioBlue

• Figure 1

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD4-FITC (B) or with the corresponding REAL Control (A). REAlease CD4-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD4-FITC (D). Cells were labeled with CD3 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were stained with REAlease CD4 Complexes or with the corresponding REAL Control (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

• Selected references

  1. Maddon, P. J. et al. (1985) The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family. Cell 42(1): 93-104
  2. Barclay, A. N. et al. (1993) CD4 and the immunoglobulin superfamily. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 342(1299): 7-12
  3. Tebas, P. et al. (2014) Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N. Engl. J. Med. 370(10): 901-910