Alternative names

Technical specifications

• Antigen: Sca-1
• Clone: REAL175
• Isotype control: REAL Control
• Alternative names of antigen: LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP
• Distribution of antigen: hematopoietic stem cells, hemotopoietic progenitor cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: FITC

• Figure 1

  Bone marrow cells from C57BL/6 mice were stained with REAlease Anti-Sca-1-FITC (B) or with the corresponding REAL Control (A). REAlease Anti-Sca-1-FITC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease Anti-Sca-1-FITC (D). Cells were labeled with CD117 antibodies in addition and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

  A:	B:
  C:	D:

• Selected references

  1. Khan, K. D. et al. (1990) Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells. Mol. Cell. Biol. 10(10): 5150-5159
  2. Bradfute, S. B. et al. (2005) Roles of Sca-1 in hematopoietic stem/progenitor cell function. Exp. Hematol. 33(7): 836-843
  3. Houlihan, D. D. et al. (2012) Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. Nat. Protoc. 7(12): 2103-2111